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ORIGINAL ARTICLE
Year : 2018  |  Volume : 9  |  Issue : 1  |  Page : 6-12

Collagen 1 alpha 2 gene polymorphism and dental fluorosis: A polymerase chain reaction study


Department of Periodontics, College of Dental Sciences, Davangere, Karnataka, India

Correspondence Address:
Dr. Kharidhi Laxman Vandana
Department of Periodontics, College of Dental Sciences, Room No 4, Davangere - 577 004, Karnataka
India
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DOI: 10.4103/srmjrds.srmjrds_84_16

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Background and Objectives: The objective of this study is to assess the association of collagen 1 alpha 2 (COL1A2) gene polymorphism in participants with dental fluorosis and periodontitis. Subjects and Methods: Atotal of 50 age- and sex-matched participants, thirty participants with dental fluorosis with or without periodontitis and 20 nonfluorosis participants with or without periodontitis, participated in this study to find the association of COL1A2 gene polymorphism, dental fluorosis, and periodontitis. Clinical parameters including plaque index, gingival bleeding index, modified gingival index, community periodontal index, and clinical attachment loss were recorded. The PvuII polymorphisms in the COL1A2 gene were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and real-time PCR. Urinary fluoride level estimation was done with fluoride ion selective electrode. Results: DNA was genotyped at the PvuII RFLP (in exon 25) marker inside the COL1A2 gene. For the PvuII RFLP, a 584-base pair (bp) DNA fragment was produced. In the gel picture after restriction digestion, only one band of 584 bp was seen in all the 50 samples. This indicates that this gene polymorphism is rare in Indian population as compared to Chinese population. Results for clinical parameters were explained using mean, standard deviation, and unpaired t-test. In addition, fluoride levels in urine were found to be significantly lower in nonfluorosis (0.48 mg/L) controls compared to cases with dental fluorosis (1.17 mg/L). Interpretation and Conclusion: This preliminary study provides no evidence of association between polymorphisms in the COL1A2 gene with dental fluorosis in high-fluoride-exposed populations. Further studies with large sample size are needed to confirm the association and the findings of this preliminary study.


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